Performance evaluation of a highfield orbitrap mass analyzer. Using an ltq orbitrap xl mass spectrometer as a testbed and modified electronics, a standard commercial orbitrap mass analyzer has been compared with a new design, featuring a thicker central. Page 75 instrument messages dialog box is displayed, which indicates whether or not the calibration procedure for an item is successful. Recently, highresolution mass spectrometry has been largely employed for compound identification, thanks to accurate mass measurements.
Pulsed q dissociation pqd for generating more fragments and extending the low mass range. Ltq xl is the only mass spectrometer that offers multiple dissociation techniques, pqd, etd and cid. If a calibration item is successful, the ltq xl ms detector saves the new calibration parameter automatically to the hard disk. Can anyone help with an orbitrap mass spectrometer. Orbitrap eclipse ms, orbitrap fusion lumos ms, orbitrap. Orbitrap, precursor ions can be dissociated in i the lit collision induced dissociation cid, both models, ii the c. Automated data dependent msn acquisition with accurate mass analysis can be effortlessly combined with rapid lcms analysis. Mellon college of science carnegie mellon university. Variants include the ltq, ltq ft circa 2003, ltq orbitrap circa 2005, ltq xl electrontransfer dissociation, etd circa.
For the first time, resolutions of greater than 50,000 fwhm and mass accuracies of 12 ppm are routinely available for ms, msms and even ms n data. Calibration sets the correct values on the mass spectrometer components to produce a proper qualitative spectrum. Schematic of the ltq orbitrap xl mass spectrometer with an hcd collision cell. Pulsed q collision induced dissociation pqd is a patented technique that eliminates the low mass cutoff concern inherent with all ion traps. Ltqxl linear iontrap ms users manual please read for training. This talk will cover the calibration, tuning, and operation of the ltqorbitrap series of instruments. This course covers the fundamentals of mass spectrometry, with emphasis on small molecule accurate mass applications. Ltq orbitrap operations 4 days training unity lab services. The ltq xl ion trap ms extends the legendary ms n performance of the ltq ion trap, incorporating more techniques to generate structural information through innovations in thermo scientific ion trap. Orbitrap eclipse ms, orbitrap fusion lumos ms, orbitrap fusion ms, and orbitrap idx ms. I usually make some cheesy joke about how we have an extensive. Welcome to the thermo scientific, ltq orbitrap xl system. As additional information, relative isotope abundance ria. Ltq orbitrap xl combining fast, sensitive linear ion trap msn with highresolution, accuratemass hram orbitrap technology, the researchproven thermo scientific ltq orbitrap xl system.
Learn more about how to operate and care for your mass spectrometer. The high mass accuracy of the ltq orbitrap velos increases the speed and con. Ions can be selected in the linear ion trap and fragmented either in the ion trap cid or in the hcd collision cell. I usually make some cheesy joke about how we have an extensive product line in lsms mass specs in total, just for life sciences lc mass spec amazing. Thermo finnigan ltq orbitrap mass spectrometer system priced to sell. It is the ultimate protein identification and biomarker discovery platform featuring unsurpassed sensitivity in ms and msn, rapid scan rate, high mass accuracy and up to 100k resolution power.
I changed the spray voltage, sheath and aux gas flow and the position of. Refurbished thermo scientific ltq orbitrap mass spectrometer complete with ion max source, esi probe, xcalibur 2. In practical traps, the outer electrode is sustained at virtual ground and a voltage of 3. All information in this guide concerning the ltq orbitrap velos also applies to the ltq orbitrap velos. Ltq orbitrap xl combining fast, sensitive linear ion trap msn with highresolution, accuratemass hram orbitrap technology, the researchproven thermo scientific ltq orbitrap xl system provides economical access to the power of hybrid ion traporbitrap analysis. Used thermo ltq orbitrap xl w etd and accela 1250 hplc.
Ltq orbitrap xl offering outstanding mass accuracy, resolving power, dynamic range, and high sensitivity the hybrid ft mass spectrometer combines a linear ion trap ms and the. Hesi source, nano spray probe, nano source housing with pulnix camera pn 31012584, xcalibur 2. The ltq orbitrap xl is a member of the family of ltq mass spectrometer ms detectors. Calibration sets the correct values on the mass spectrometer components to produce a proper. All information in this guide concerning the ltq orbitrap velos also applies to the ltq orbitrap velos etd system where the etd module is physically coupled to the back of the ltq orbitrap velos. Identify, characterize and quantify proteins and peptides using the thermo scientific ltq orbitrap xl etd hybrid ion trap orbitrap mass spectrometer. We are not sure if this is a hardware or software issue. Learn more about how to operate and care for your mass. As a result, the resolving power at mz 400 and 768 ms detection time can range from 60,000 for a standard trap at 3. Shotgun proteomics has made great advances due to the improved sensitivity, resolution, and speed of recent generations of mass spectrometers 1, 2. Ltq orbitrap xl mass spectrometer we know you, or someone you know, worked very hard to get your orbitrap based mass spectrometer in your lab and even harder preparing your precious samples. Significantly, the orbitrap xl etd includes two fragmentation regimes, higherenergy ctrap dissociation hcd and electron transfer dissociation etd, that are complementary to the ltqs.
Ions generated by api are collected in the ltq xl followed by axial ejection to the cshaped storage trap which is used to store and collisionally. Nanoviper fitting technology enables low dead volume liquid flow path connectivity. This instrument is a comprehensive solution for a range of proteomics applications including complex ptm analysis. This course covers the fundamentals of mass spectrometry, with emphasis on small molecule. Achieving peak performance from your velos pro, orbitrap. Orbitrap tribrid ms series instrument control software version 3. The thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer combines highperformance quadrupole precursor selection with highresolution, accuratemass hram orbitrap. Thermo scientific ltq xl getting started pdf download. A tube lens of 110 v ltq orbitrap xl instrument or slens of 50% ltq orbitrap velos instrument was used in combination with 10 v of source voltage.
The thermo ltq is a 2d linear ion trap ms, first released circa 2003. For the ltq orbitrap velos, the mass spectrum is acquired with an orbitrap, and tandem mass spectrometry msms. Can anyone help with an orbitrap mass spectrometer calibration issue. Procedures in chapters 14 can be performed from the. The ltq orbitrap velos and qexactive are two widely used mass spectrometers for largescale proteomic analysis.
The first commercial instrument to utilize this capability, ltq orbitrap classic, was introduced by thermo electron currently thermo fisher scientific in 2005 and was followed by important extensions of the same family. Computer dell xeon system loaded with windows 7, xcalibur 2. Thermo fisher orbitrap instrument control software. This instrument is a comprehensive solution for a range. Communicate more effectively with us if there is a problem that cannot be solved using this troubleshooting guide. Achieve peak performance with your velos pro, orbitrap velos pro, or orbitrap elite. Ltq orbitrap velos is a member of the family of ltq mass spectrometer ms hybrid instruments. Once you have your skyline document open on the ltq instrument control computer, you are ready to export it to a native ltq method or. Can anyone help with an ltq orbitrap xl mass spectrometer. It is the ultimate protein identification and biomarker discovery. Ltq orbitrap xl system uf icbr university of florida. Ltq orbitrap xl mass spectrometer a superior alternative to qtof systems.
Scope of this guide this ltq orbitrap xl getting started manual provides you with information on how to set up, calibrate, and tune the ltq orbitrap xl. Thermo finnigan ltq orbitrap ms eci source powervar series 2000 gpi global power interface 2. Ltq orbitrap xl offering outstanding mass accuracy, resolving power, dynamic range, and high sensitivity the hybrid ft mass spectrometer combines a linear ion trap ms and the orbitrap mass analyzer. Ltq orbitrap xl getting started includes the following chapters. The new maldi source, available on the thermo scientific ltq orbitrap xl and ltq orbitrap discovery, is ideally suited for proteomic and metabolism applications. The ltq orbitrap xl features an hcd collision cell to provide additional. Thermo finnigan ltq orbitrap mass spectrometer system. I was calibrating our ltqorbitrap xl and i noticed very unstable esi spray 40% rsd in the api stability evaluation. Click the browse button beside the template file field.
The ltq orbitrap is a high performance lcms and msn system, combining rapid ltq ion trap data acquisition with high mass accuracy orbitrap mass analysis. The ltq xl ion trap ms extends the legendary ms n performance of the ltq ion trap, incorporating more techniques to generate structural information through innovations in thermo scientific ion trap technology unparalleled sensitivity and unmatched ms n spectral quality. Equipped with ion max source and dual edwards e2m30 vacuum pumps, as well as various accessories as shown. Tuning adjusts the voltages on the source and ion optics to maximize the instruments sensitivity. Ltq orbitrap velos hardware manual brown university. Ltq orbitrap operations 4 days this course is intended for new users to the thermo scientific orbitrap system. Automatic calibration of all transfer and orbitrap parameters via instrument control software ltq xl the ltq xl ion trap ms extends the legendary msnperformance of the ltq ion trap, incorporating more. About this guide this ltq orbitrap xl getting started manual provides you with information on how to set up, calibrate, and tune the ltq orbitrap xl. Pulsed q collision induced dissociation pqd is a patented technique that eliminates the low mass. We are working just as hard to make sure that you have the latest information and all the additional resources you need to produce the highest quality data. The superior quality of q exactive msms data provides ultimate confidence for a wide range of qualitative and quantitative applications. Software integration with ms and instrument control using xcalibur 4.
I was calibrating our ltq orbitrap xl and i noticed very unstable esi spray 40% rsd in the api stability evaluation. Used thermo scientific ltq orbitrap xl for sale id. The thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer combines highperformance quadrupole precursor selection with highresolution, accuratemass hram orbitrap detection. Thisthermo ltq orbitrap ms system is in excellent condition. The standard higherenergy collisional dissociation hcd collision cell provides exceptional flexibility in. Mass spectrometer thermo scientific ltq xl with orbitrap xl. Troubleshooting ltq and ltqorbitrap lcmsms systems with. The first commercial instrument to utilize this capability, ltq orbitrap classic, was introduced by thermo electron currently thermo fisher scientific in 2005 and was followed by important extensions of the. Currently the orbitrap analyzer exists in two variants.
Ltq orbitrap xl mass spectrometer we know you, or someone you know, worked very hard to get your orbitrapbased mass spectrometer in your lab and even harder preparing your precious samples. Identify, characterize and quantify proteins and peptides using the thermo scientific ltq orbitrap xl etd hybrid ion traporbitrap mass spectrometer. While viewing total ion current or base peak intensity may or may not shed light on these problems, massqc alerts you to outofrange metrics through robust trend. Ltq xl getting started thermo electron corporation. Nano spray probe, michrom bioresources captivespray ion source pn ss20000300 thermo cs, ltq xl, ft, orbitrap, xcalibur 2. The new ltq orbitrap xl hybrid ft mass spectrometer combines a linear ion trap ms and the orbitrap mass analyzer. Procedures in chapters 14 can be performed from the xcalibur tune plus window.
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